Rapid Kit For HCV Nucleic Acid Detection And Its Detection Method 1
Chemtron biotech 's present invention discloses a kit for rapid step detection of HCV nucleic acid, including nucleic acid extract, rt-qpcr reaction fluid, negative control, positive control and positive reference material. The present invention mainly solves the longtime disadvantage of testing nucleic acid of existing kits on the market. The hcv rapid test kit , based on the basic reaction principle of conventional rt-qpcr, realizes nucleic acid denaturation at a lower temperature by changing the composition of the reaction buffer and the primer, the unique design and synthesis of the probe, thus shortening the time difference of denaturation and temperature-restoring difference of PCR, and greatly shortening the whole reaction time. The kit of the present invention has high detection sensitivity, specificity and repeatability, and can detect six common genotypes of hepatitis c virus. Meanwhile, it is also suitable for regular quantitative PCR instrument, which provides a...